Name of the infrastructure: ICN-TA

Location (town, country): Barcelona (Spain)

Web site address: www.nanocat.org; www.cin2.es/english/index.php

Legal name of organisation operating the infrastructure: CIN2 Centre in Nanoscience and Nanotechnology(ICN-CSIC): Institut Català de Nanotecnologia-Consejo Superior de Investigaciones Científicas

Location of organisation (town, country): Barcelona, Spain


Description of the Infrastructure


The Research Centre in Nanoscience and Nanotechnology (CIN2) is sponsoredby the Superior Council of Scientific Research (CSIC) and the Catalan Institute of Nanotechnology (ICN). Theobjective is excellence in Nanoscience and Nanotechnology. Several research lines have been developed tocover every aspect of Nanotechnology, and to encompass the multidisciplinarity of the field. CIN2 researchersare especially active at the international level with collaboration in numerous European projects, publications inhigh impact journals, innovation (with Spin-off companies like Sensia and Endor), teaching and social awarenessof science.

The group has obtained considerable international recognition in synthesis and potential biomedical applicationsof nanoparticles. Dr. Puntes also has experience in the coordination of multidisciplinary and multi-institutionalprojects, and dedicates special attention to the popularisation and dissemination of new achievements andchallenges in Nanotechnology. The laboratory is specialist in synthesis and derivation of engineered inorganicnanoparticles and their interaction with biological environments, including synthesis, physical and chemicalcharacterization of nanoparticles before and after exposure to cell cultures and cell culture media and thestability of those nanoparticles, including aggregation, disintegration (corrosion), and surface state modifications.The infrastructure will be opened to train anyone to prepare and characterize their own nanoparticles or in theuse of nanoparticles provided by CIN2. In addition, manipulation, handling and disposal of NPs protocols will beimplemented and standardized.



CIN2 will be offering access to three of the components listed inthe project:


Particle Synthesis:

Wet synthesis lab: An experienced lab specially suited to perform the synthesis of inorganic nanoparticles withextensive knowledge in the development of synthesis protocols for inorganic nanoparticles. A large variety ofreference materials can be offered to the community: Au spheres (4 – 40 nm), rods, cubic structures, tubes; Agspheres, rods, cubes; Pt spheres, multipods, cubes; Pd spheres, cubic structures; Fe spheres; Fe3O4 spheres;Fe2O3 spheres, hollow spheres; Co spheres, disks; CoO spheres, hollow spheres; CeO2 spheres, belts;TiO2 spheres; ZnO spheres; CoPt spheres, Co-organic-Au (spheres also include cuboctahedron or shapelessparticles). The facility is equipped with glove box, laminar flow hoods, fume cupboards and the required facilitiesto avoid contamination in nanoparticle synthesis. The analysis equipment in-house also includes DLS and Z-potential (Malvern Z-sizer) and a Transmission Electron Microscope 200 kV (soon available). For QNano, theparticles that will be offered are Fe spheres; Fe3O4 spheres; Fe2O3 spheres, hollow spheres, CeO2 spheres,belts; TiO2 spheres; ZnO spheres mainly at the existing in the research lab scale. These facilities are supportedby 1 technical support personnel.



Conjugation lab: The conjugation of these NPs is performed with a large variety of organic (polar, non-polar),inorganic (cisplatin), and biochemical components (proteins, peptides, RNA, DNA and other biomolecules). Thelab is capable of producing moderate-scale, high quality and stable samples. Analytical facilities to carry outqualitative and quantitative analysis of a wide range of samples from environmetal and biological areas are alsoavailable: ICP-MS, FT-IR (PM-IRRAS, Bruker), centrifuges, etc


Particle characterisation:

Analytical, imaging and structural characterisation: These include recently acquired (or to be purchased in thevery near future). TEM (EDS, EELS, HAADF, STEM) 200 kV, SEM (H.RES and Environmental) MAGELLAN(FEI) + Hitachi H-4100FE + QUANTA FEG, Optical microscopes Axio Observer Z1m (Zeiss), XRD (equippedwith SAXS) PANalytical X´Pert, DLS and Z-Potential (Malvern), UV-VIS spectroscopy, Atomic Force Microscopy(AFM) Scientec + Veeco (DI). These facilities are supported by 3 technical support personnel. In addition, ICNalso offers training and expertise in the characterisation of inorganic nanoparticles in organic and biologicalenvironments.


Research supported by the infrastructure

ICN research is organised into a framework with 6 specific foci: 1. Atomic Manipulation and Spectroscopy, 2.Inorganic nanoparticle synthesis, 3. Magnetic Nanostructures, 4. Nanobioelectronics & Biosensors, 5. Phononicand Photonic Nanostructures, 6. Physics and Engineering of Nanoelectronic Devices.


Services currently on offer and scientific highlights

CIN2 (ICN-CSIC) offers a dynamic interdisciplinary research environment, the particular strength of which isthe close collaboration of scientists from a range of disciplines, specifically material scientists, physical andbiophysical scientists, chemists, molecular biologists as well as the availability of a full technical support whenaccessing analytical equipment.