Name of the infrastructure: UU-TA

Location (town, country): Uppsala, Sweden

Web site address: www.myfab.se, www.uu.se

Legal name of organisation operating the infrastructure: Uppsala University

Location of organisation (town, country): Uppsala, Sweden


Description of the Infrastructure


Ångström Microstructure Laboratory (UU) is a multidisciplinary facility with resources for research anddevelopment in materials science and nanotechnology. Applications can be found in life science, biotechnologyand medical diagnostics. Available resources include 2000 m2 of cleanroom area, 150 instruments and atechnical staff of 10 skilled professionals. Tools are provided for conventional processing, such as lithography,various wet and dry etching processes, physical and chemical deposition and high temperature processes.The key component of the UU offer is the analytical facilities with state-of-the-art electron microscopy, X-rayphotoelectron spectroscopy, various ion beam analysis techniques and more. The scientific expertise covers awide field with materials science, chemistry, nanotechnology, medicine and pharmacy. High resolution analyticalmicroscopy is a strong research area for UU, with comprehensive and state-of-the-art equipment. Nanoparticleanalysis capabilities include crystallographic structure, chemical composition (including gradients, core shellstructures, etc.), chemical bonding states, morphology, in situ behaviour (sub-micrometer resolution) under heattreatments, gases and moist/wet conditions. Novel methods of using the analysis techniques for in vivo sampleshave also been developed.

UU is a dynamic meeting place with 200 external and internal users from academic research as well as industrialdevelopment. All users get a general safety introduction and individual training on specific tools needed for theirwork.

The UU-TA infrastructure operates as an open User facility, where researchers and engineers (from universitiesand industry) can get training and access to the available resources. Technical expertise and methodologycompetence is normally offered by the lab staff, whereas more application specific expertise can be provided bythe internal research groups active within the infrastructure.



UU will be offering access to various components as follows:


Particle Synthesis:

Biomimetic growth of nanoparticles.


Particle Characterisation:

The UU analysis facility includes a number of tools for scanning electron microscopy (SEM), includingFEG-SEM, variable pressure SEM and focused ion beam (FIB). Most of these tools are equipped withenergy-dispersive spectrometry (EDS) for elemental analysis. Two instruments are available for transmission electron microscopy (TEM), including a 300 kV high-resolution TEM/STEM with EDS, EELS, HAADF andbiprism. Chemical surface analysis is provided by X-ray photoelectron spectroscopy (XPS). Additional toolsinclude atomic force microscopy (AFM), state-of-the-art nanoindenter, optical profilometry, optical microscopyand a well equipped laboratory for sample preparation. A 5 MV tandem accelerator is available for variousion beam analysis techniques (RBS, NRA, ERDA). Access to particle size determination techniques, such asMalvern, can be provided.


Particle Exposure Assessment:

Analytical techniques for particle characterisation from in vitro and in vivo experiments, e.g. SEM, TEM and FIB.


Research supported by the infrastructure

Nanosafety is directly related to the uptake and adverse reactions of particles in human tissue. With thedesign of new nanomaterials or nanoassemblies, the relation between nanoparticle structure and reactionsin-vivo is the key for understanding behaviour. Basic studies involve synthesis of technological relevantstructures, intermediate screening in-vitro and functional tests in-vivo. Of special importance is to not freelyextrapolate in-vitro data to in-vivo, but rather to correlate these. Technologically this basic area has relevance fornanoparticle “pollutants” and nanoparticle “medicine”.


Services Currently on Offer and Scientific Highlights

Ongoing research activities in the nano-biomedical field include• Implantable nanostructured biomaterials• Inorganic drug delivery systems (nanosized or nanoporous)• Nanoparticles for diagnostical applicationsA strong area, with comprehensive and state-of-the-art equipment, is high resolution analytical microscopy.Nanoparticle analysis capabilities include crystallographic structure, chemical composition (including gradients,core shell structures, etc.), chemical bonding states, morphology, in situ behaviour (sub-micrometer resolution)under heat treatments, gases and moist/wet conditions. Novel methods of using the analysis techniques for invivo samples have also been developed.