Joint Research Activity 4 - Optimal modes of presentation of nanoparticles to cells, tissues, organisms and animals

The key objectives are:

  • Comparison of various methods of presentation of nanoparticles to cells (e.g. from dispersion, following deposition on surfaces of varying stickiness, with agitation or flow) on the quantitative reproducibility of nanoparticle uptake and distribution across the cellular population for adherent and non-adherent cell types;
  • Delivery to TA of protocols for optimal method of presentation of nanoparticles for various cell types (adherent and non-adherent) for in vitro studies;
  • Comparison of various methods of presentation of nanoparticles for in vivo studies (PBS dispersion, bolus in food, plasma dispersion) in terms of the dose delivered and the time-resolved aggregation of the particles from each method;
  • Recommendations regarding the optimal method of presentation of nanoparticles for various cell types (adherent and non-adherent) for in vivo studies.